High speed image processing system and its micro optics application

Kohtaro Ohba; Jesus Carlos Pedraza Ortega; Tamio Tanikawa; Kazuo Tanie; Kenji Tajima; Hiroshi Nagai; Masataka Tsuji; Shigeru Yamada

doi:10.1117/12.516776

1 August 2003 High-speed image processing system and its micro-optics application

Kohtaro Ohba, Jesus Carlos Pedraza Ortega, Tamio Tanikawa, Kazuo Tanie, Kenji Tajima, Hiroshi Nagai, Masataka Tsuji, Shigeru Yamada

Author Affiliations +

Proceedings Volume 4948, 25th International Congress on High-Speed Photography and Photonics; (2003) https://doi.org/10.1117/12.516776
Event: 25th international Congress on High-Speed photography and Photonics, 2002, Beaune, France

Abstract

In this paper, a new application system with high speed photography, i.e. an observational system for the tele-micro-operation, has been proposed with a dynamic focusing system and a high-speed image processing system using the "Depth From Focus (DFF)" criteria. In micro operation, such as for the microsurgery, DNA operation and etc., the small depth of a focus on the microscope makes bad observation. For example, if the focus is on the object, the actuator cannot be seen with the microscope. On the other hand, if the focus is on the actuator, the object cannot be observed. In this sense, the "all-in-focus image," which holds the in-focused texture all over the image, is useful to observe the microenvironments on the microscope. It is also important to obtain the "depth map" which could show the 3D micro virtual environments in real-time to actuate the micro objects, intuitively. To realize the real-time micro operation with DFF criteria, which has to integrate several images to obtain "all-in-focus image" and "depth map," at least, the 240 frames par second based image capture and processing system should be required. At first, this paper briefly reviews the criteria of "depth from focus" to achieve the all-in-focus image and the 3D microenvironments' reconstruction, simultaneously. After discussing the problem in our past system, a new frame-rate system is constructed with the high-speed video camera and FPGA hardware with 240 frames par second. To apply this system in the real microscope, a new criterion "ghost filtering" technique to reconstruct the all-in-focus image is proposed. Finally, the micro observation shows the validity of this system.

Citation Download Citation

Kohtaro Ohba, Jesus Carlos Pedraza Ortega, Tamio Tanikawa, Kazuo Tanie, Kenji Tajima, Hiroshi Nagai, Masataka Tsuji, and Shigeru Yamada "High-speed image processing system and its micro-optics application", Proc. SPIE 4948, 25th International Congress on High-Speed Photography and Photonics, (1 August 2003); https://doi.org/10.1117/12.516776

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microscopes

Image processing

Imaging systems

Cameras

Field programmable gate arrays

Image quality

Video

Show All Keywords

Keywords/Phrases

Search In:

Publication Years