Effects of tissue optical properties on time-resolved fluorescence measurements from brain tumors: an experimental and computational study

Pramod V. Butte; Karthik Vishwanath; Brian K. Pikul; Mary-Ann Mycek; Laura Marcu

doi:10.1117/12.478192

29 July 2003 Effects of tissue optical properties on time-resolved fluorescence measurements from brain tumors: an experimental and computational study

Pramod V. Butte, Karthik Vishwanath, Brian K. Pikul, Mary-Ann Mycek, Laura Marcu

Author Affiliations +

Proceedings Volume 4955, Optical Tomography and Spectroscopy of Tissue V; (2003) https://doi.org/10.1117/12.478192
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Time-Resolved Laser-Induced Fluorescence Spectroscopy (tr-LIFS) offers the potential for intra-operative diagnosis of primary brain tumors. However, both the intrinsic properties of endogenous fluorophores and the optical properties of brain tissue could affect the fluorescence measurements from brain. Scattering has been demonstrated to increase, for instance, detected lifetimes by 10-20% in media less scattering than the brain. The overall goal of this study is to investigate experimentally and computationally how optical properties of distinct types of brain tissue (normal porcine white and gray matter) affect the propagation of the excitation pulse and fluorescent transients and the detected fluorescence lifetime. A time-domain tr-LIFS apparatus (fast digitizer and gated detection) was employed to measure the propagation of ultra-short pulsed light through brain specimens (1-2.5-mm source-detector separation; 0.100-mm increment). A Monte Carlo model for semi-infinite turbid media was used to simulate time-resolved light propagation for arbitrary source-detector fiber geometries and optical fiber specifications; and to record spatially- and temporally resolved information. We determined a good correlation between experimental and computational results. Our findings provide means for quantification of time-resolved fluorescence spectra from healthy and diseased brain tissue.

Citation Download Citation

Pramod V. Butte, Karthik Vishwanath, Brian K. Pikul, Mary-Ann Mycek, and Laura Marcu "Effects of tissue optical properties on time-resolved fluorescence measurements from brain tumors: an experimental and computational study", Proc. SPIE 4955, Optical Tomography and Spectroscopy of Tissue V, (29 July 2003); https://doi.org/10.1117/12.478192

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