Time-resolved spectroscopic and microscopic measurements of membrane dynamics in living cells

Herbert Schneckenburger; Martina Kretzschmar; Michael Wagner; Wolfgang S. L. Strauss; Reinhard Sailer

doi:10.1117/12.478118

19 June 2003 Time-resolved spectroscopic and microscopic measurements of membrane dynamics in living cells

Herbert Schneckenburger, Martina Kretzschmar, Michael Wagner, Wolfgang S. L. Strauss, Reinhard Sailer

Proceedings Volume 4962, Manipulation and Analysis of Biomolecules, Cells, and Tissues; (2003) https://doi.org/10.1117/12.478118
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Fluorescence spectra and lifetime images of cultivated CHO cells incubated with the membrane marker laurdan are reported. The plasma membrane and intracellular membranes were distinguished using illumination of either the cell surface (by an evanescent wave) or of the whole cell. Parameters of membrane dynamics, probed by the generalized prolarization and the effective fluorescence lifetime of laurdan, were correlated with temperature, age and growth phase of the cells.

Citation Download Citation

Herbert Schneckenburger, Martina Kretzschmar, Michael Wagner, Wolfgang S. L. Strauss, and Reinhard Sailer "Time-resolved spectroscopic and microscopic measurements of membrane dynamics in living cells", Proc. SPIE 4962, Manipulation and Analysis of Biomolecules, Cells, and Tissues, (19 June 2003); https://doi.org/10.1117/12.478118

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