The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes

Lisong Yang; Cian M. Taylor; Yury Rakovich; Eithne M. McCabe

doi:10.1117/12.477978

9 July 2003 The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes

Lisong Yang, Cian M. Taylor, Yury Rakovich, Eithne M. McCabe

Proceedings Volume 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X; (2003) https://doi.org/10.1117/12.477978
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Images of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.

Citation Download Citation

Lisong Yang, Cian M. Taylor, Yury Rakovich, and Eithne M. McCabe "The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes", Proc. SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X, (9 July 2003); https://doi.org/10.1117/12.477978

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