Single-cell analysis on a microchip platform using optical tweezers and optical scissors

Nigel R. Munce; Jianzhao Li; Peter R. Herman; Lothar D. Lilge

doi:10.1117/12.472892

17 January 2003 Single-cell analysis on a microchip platform using optical tweezers and optical scissors

Nigel R. Munce, Jianzhao Li, Peter R. Herman, Lothar D. Lilge

Proceedings Volume 4982, Microfluidics, BioMEMS, and Medical Microsystems; (2003) https://doi.org/10.1117/12.472892
Event: Micromachining and Microfabrication, 2003, San Jose, CA, United States

Abstract

Microfluidic devices, or "lab-on-a-chip" systems for single cell analysis represent a new field of micro-total analysis systems (μTAS) that could not only perform a task quicker, and more accurately than conventional methods, but could also incorporate additional tools to the study of biological variability in a population by allowing researchers to directly examine the contents of a multitude of single cells from the population under study. Realizing such a device, presents several engineering challenges to the fields of micro-machining, micromanipulation and analytical bio-chemistry. The device needs to be able to accurately and efficiently select, manipulate and analyze volumes represented by a single cell without diluting the contents. For this purpose, optical tweezers and scissors were implemented to select single cells on a microchip, bring the cell to a desired location, and lyse the cell using the optical scissors. Channels were engineered in the device using a molecular fluorine (F₂) laser. Each channel’s cross-section is approximately the size of an individual cell (10μm wide and deep). This paper, describes the manipulation of cells on a microchip using optical tweezers and the injection of the cellular contents by optical scissors from a single cell into 10μm channels.

Citation Download Citation

Nigel R. Munce, Jianzhao Li, Peter R. Herman, and Lothar D. Lilge "Single-cell analysis on a microchip platform using optical tweezers and optical scissors", Proc. SPIE 4982, Microfluidics, BioMEMS, and Medical Microsystems, (17 January 2003); https://doi.org/10.1117/12.472892

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available