Light propagation in nonaggregating RBC suspension: Monte Carlo simulation and comparison with experiment

Mikhail Yu. Kirillin; Vladimir V. Lopatin; Alexander V. Priezzhev

doi:10.1117/12.518794

13 October 2003 Light propagation in nonaggregating RBC suspension: Monte Carlo simulation and comparison with experiment

Mikhail Yu. Kirillin, Vladimir V. Lopatin, Alexander V. Priezzhev

Author Affiliations +

Proceedings Volume 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV; (2003) https://doi.org/10.1117/12.518794
Event: Saratov Fall Meeting 2002 Laser Physics and Photonics, Spectroscopy, and Molecular Modeling III; Coherent Optics of Ordered and Random Media III, 2002, Saratov, Russian Federation

Abstract

In this work we used angle-resolved Monte-Carlo simulation to study light propagation in non-aggregated RBC suspensions. In our model we used phase functions, calculated in different optic approximation (geometrical optics, Fraunhofer diffraction, hybrid approximation, Mie theory) as well Heyney-Greenstein function. Scattering indicatrices obtained in different approximations were compared. Heyney-Greenstein function was used for calculations at different hematocrits. It was shown that for hematocrits in a range of 20...50 % the scattering indicatrices for wavelength 633 nm do not differ significantly, however this difference is higher fro smaller hematocrit. We also compared contributions of single and multiple scattering to light intensity distribution at different angles. We showed that for 100-μm thick suspension layer the major contribution to forward scattering is given by snake-photons, while to backward scattering - by multiply scattered photons.

Citation Download Citation

Mikhail Yu. Kirillin, Vladimir V. Lopatin, and Alexander V. Priezzhev "Light propagation in nonaggregating RBC suspension: Monte Carlo simulation and comparison with experiment", Proc. SPIE 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV, (13 October 2003); https://doi.org/10.1117/12.518794

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