Measurement of changes in concentrations of biological solutions using a Rayleigh interferometer

Y. C. Chen; J. J. Brazier; Sean J. Kirkpatrick; Scott A. Prahl

doi:10.1117/12.518786

13 October 2003 Measurement of changes in concentrations of biological solutions using a Rayleigh interferometer

Y. C. Chen, J. J. Brazier, Sean J. Kirkpatrick, Scott A. Prahl

Proceedings Volume 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV; (2003) https://doi.org/10.1117/12.518786
Event: Saratov Fall Meeting 2002 Laser Physics and Photonics, Spectroscopy, and Molecular Modeling III; Coherent Optics of Ordered and Random Media III, 2002, Saratov, Russian Federation

Abstract

A Rayleigh interferometer was constructed to measure changes of concentrations in the biological solutions. With the stability tests, our Rayleigh interferometer system showed its insensitivity to environment vibrations and with the second compensating cuvette, effects on the refractive index changes other than the concentration changes of molecules in the sample solution could be compensated. A thin glass plate was inserted in the beam path and rotated to vary the optical path length to test the sensitivity of the system. With this glass plate, the detectable optical path differences of the system was Δ(nl) = 7 nm. Finally, the concentration of sucrose solutions were varied to change the refractive index. The refractive index changes by 1.43 × 10^-4for each gram of sucrose per liter at 20°C. With our system, the sensitivity to sucrose solution was 7mg/L. Based on this sensitivity this interferometric system can be used to detect concentrations of albumin solutions as low as 0.6mg/mL.

Citation Download Citation

Y. C. Chen, J. J. Brazier, Sean J. Kirkpatrick, and Scott A. Prahl "Measurement of changes in concentrations of biological solutions using a Rayleigh interferometer", Proc. SPIE 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV, (13 October 2003); https://doi.org/10.1117/12.518786

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available