Methylation oligonucleotide microarray: a novel tool to analyze methylation patterns

Peng Hou; Meiju Ji; Nongyao He; Zuhong Lu

doi:10.1117/12.497693

18 April 2003 Methylation oligonucleotide microarray: a novel tool to analyze methylation patterns

Peng Hou, Meiju Ji, Nongyao He, Zuhong Lu

Proceedings Volume 5119, Bioengineered and Bioinspired Systems; (2003) https://doi.org/10.1117/12.497693
Event: Microtechnologies for the New Millennium 2003, 2003, Maspalomas, Gran Canaria, Canary Islands, Spain

Abstract

A new technique to analyze methylation patterns in several adjacent CpG sites was developed and reported here. We selected a 336bp segment of the 5’-untranslated region and the first exon of the p16Ink4a gene, which include the most densely packed CpG fragment of the islands containing 32 CpG dinucleotides, as the investigated target. The probes that include all types of methylation patterns were designed to fabricate a DNA microarray to determine the methylation patterns of seven adjacent CpG dinucleotides sites. High accuracy and reproducibility were observed in several parallel experiments. The results led us to the conclusion that the methylation oligonucleotide microarray can be applied as a novel and powerful tool to map methylation patterns and changes in multiple CpG island loci in a variety of tumors.

Citation Download Citation

Peng Hou, Meiju Ji, Nongyao He, and Zuhong Lu "Methylation oligonucleotide microarray: a novel tool to analyze methylation patterns", Proc. SPIE 5119, Bioengineered and Bioinspired Systems, (18 April 2003); https://doi.org/10.1117/12.497693

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