EnFET for urea determination in biological fluids using ammonium ion detection

Marek Dawgul; Tomasz Trybun; Dorota G. Pijanowska; Wladyslaw Torbicz

doi:10.1117/12.517106

22 September 2003 EnFET for urea determination in biological fluids using ammonium ion detection

Marek Dawgul, Tomasz Trybun, Dorota G. Pijanowska, Wladyslaw Torbicz

Proceedings Volume 5124, Optoelectronic and Electronic Sensors V; (2003) https://doi.org/10.1117/12.517106
Event: Optoelectronic and Electronic Sensors V, 2002, Rzeszow, Poland

Abstract

In this paper a method of urease immobilization on the surface of the Siloprene membrane of the ammonium ion sensitive ChemFET is presented. The usability of the sensor for determination of urea in solutions at pH typical for biological fluids (pH 6 to pH 7.5) has been investigated. Due to the fact that the sensor exhibits high sensitivity to samples of low buffer capacity, the method of preliminary sample treatment, consisting in addition of buffers at adequate buffer capacity was developed. The sensors were tested in dialysate and blood plasma.

Citation Download Citation

Marek Dawgul, Tomasz Trybun, Dorota G. Pijanowska, and Wladyslaw Torbicz "EnFET for urea determination in biological fluids using ammonium ion detection", Proc. SPIE 5124, Optoelectronic and Electronic Sensors V, (22 September 2003); https://doi.org/10.1117/12.517106

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