Combined effect of multiple light scattering and radiative transport on fluorescence emission

Sandrina P Barbosa; Aleksander Fedorov; Eduardo N Pereira; Jose M. G. Martinho; Mario N Berberan-Santos

doi:10.1117/12.500711

15 October 2003 Combined effect of multiple light scattering and radiative transport on fluorescence emission

Sandrina P Barbosa, Aleksander Fedorov, Eduardo N Pereira, Jose M. G. Martinho, Mario N Berberan-Santos

Proceedings Volume 5138, Photon Migration and Diffuse-Light Imaging; (2003) https://doi.org/10.1117/12.500711
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

The combined effect of multiple light scattering and radiative transport on fluorescence decays is experimentally studied. The experimental setup consists of a 15 cm cubic cell containing a highly fluorescent dye solution and scatterers (either silica particles or milk). The effect of fluorophore concentration was studied, in the absence and presence of scatterer. The effect of excitation and detection geometry was also studied. It is concluded that light scattering can have a significant effect on the fluorescence decays, namely on the mean decay times, and the results can be explained on the basis of the spatial distributions of the successive generations of excited molecules.

Citation Download Citation

Sandrina P Barbosa, Aleksander Fedorov, Eduardo N Pereira, Jose M. G. Martinho, and Mario N Berberan-Santos "Combined effect of multiple light scattering and radiative transport on fluorescence emission", Proc. SPIE 5138, Photon Migration and Diffuse-Light Imaging, (15 October 2003); https://doi.org/10.1117/12.500711

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available