Measurement of mammalian erythrocyte indices from light scattering with scanning flow cytometer

Konstantin A. Semianov; Peter Alexandrovich Tarasov

doi:10.1117/12.500632

8 October 2003 Measurement of mammalian erythrocyte indices from light scattering with scaning flow cytometer

Konstantin A. Semianov, Peter Alexandrovich Tarasov

Proceedings Volume 5141, Diagnostic Optical Spectroscopy in Biomedicine II; (2003) https://doi.org/10.1117/12.500632
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

The technique of scanning flow cytometery (SFC) was adopted for measurement of erythrocyte indices: volume, hemoglobin concentration and surface area. The method has been verified on two types of mammalian red cells: human and murine. In order to access distribution of values, precision and stability of inverse algorithm of reconstitution of size and refractive index of microsphere from light scattering angular dependency (indicatrix) have been increased performing analysis of Fourier spectrum of indicatrix. Volume - hemoglobin concentration (V-HbC) map was obtained following well-known procedure of isovolumetric sphering, presented by Technicon Instruments. Additionally new method for measurement of paired distribution of erythrocyte surface area (S) and hemoglobin content (Hb) was implemented via registration of spherical stage in the course of colloid-osmotic hemolysis. Approach to characterization with SFC of native red cells in nonspherical stage is demonstrated.

Citation Download Citation

Konstantin A. Semianov and Peter Alexandrovich Tarasov "Measurement of mammalian erythrocyte indices from light scattering with scaning flow cytometer", Proc. SPIE 5141, Diagnostic Optical Spectroscopy in Biomedicine II, (8 October 2003); https://doi.org/10.1117/12.500632

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available