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Raman spectroscopy has been employed to measure the varying concentrations of two oral bacteria in simple mixtures. Evaporated droplets of centrifuged mixtures of Streptococcus sanguis and Streptococcus mutans were analyzed via Raman microspectroscopy. The concentration of s. sanguis was determined based upon the measured Raman spectrum, using partial least squares cross-validation, with an r2 value of 0.98.
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Andrew J. Berger, Qingyuan Zhu, Robert G. Quivey, "Raman spectroscopy of oral bacteria," Proc. SPIE 5141, Diagnostic Optical Spectroscopy in Biomedicine II, (8 October 2003); https://doi.org/10.1117/12.500953