Control of single-molecule fluorescence dynamics by stimulated emission depletion

Richard J. Marsh; Mark A. Osborne; Angus J. Bain

doi:10.1117/12.505861

14 November 2003 Control of single-molecule fluorescence dynamics by stimulated emission depletion

Richard J. Marsh, Mark A. Osborne, Angus J. Bain

Proceedings Volume 5222, Nanocrystals, and Organic and Hybrid Nanomaterials; (2003) https://doi.org/10.1117/12.505861
Event: Optical Science and Technology, SPIE's 48th Annual Meeting, 2003, San Diego, California, United States

Abstract

The feasibility of manipulating the single molecule absorption-emission cycle using picosecond stimulated emission depletion (STED) is investigated using a stochastic computer simulation. In the simulation the molecule is subjected to repeated excitation and depletion events using time delayed pairs of excitation (PUMP) and depletion (DUMP) pulses derived from a high repetition rate pulsed laser system. The model is used to demonstrate that a significant and even substantial reduction in the occurrence of 'dark states' in the fluorescence emission can be achieved using stimulated emission depletion. Variation in the PUMP-DUMP window allows precise control of the fluorescence yield with substantial increases in the fluorescence intensity observed at early PUMP-DUMP delays.

Citation Download Citation

Richard J. Marsh, Mark A. Osborne, and Angus J. Bain "Control of single-molecule fluorescence dynamics by stimulated emission depletion", Proc. SPIE 5222, Nanocrystals, and Organic and Hybrid Nanomaterials, (14 November 2003); https://doi.org/10.1117/12.505861

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