Fluorometric determination of whole blood with various excitation wavelengths

Xiufeng Lan; Shumei Gao; Changde Peng; Ying Liu; Xiao-Wu Ni

doi:10.1117/12.546092

8 December 2003 Fluorometric determination of whole blood with various excitation wavelengths

Xiufeng Lan, Shumei Gao, Changde Peng, Ying Liu, Xiao-Wu Ni

Proceedings Volume 5254, Third International Conference on Photonics and Imaging in Biology and Medicine; (2003) https://doi.org/10.1117/12.546092
Event: Third International Conference on Photonics and Imaging in Biology and Medicine, 2003, Wuhan, China

Abstract

Autofluorescence spectra from whole blood of laboratory rat are measured in this paper. The excitation lights are light emitting diode (LED), Ar⁺ laser, and He-Ne laser with the wavelength located at 457nm, 457.9nm, and 632.8nm respectively. The three spectral profiles are found to be substantially different, each displaying its own characteristic fluorescence bands. Ar⁺ laser-induced spectrum has very rich and sharp peaks. The LED-induced one has the strongest and widest fluorescence bands. And the intensity of the spectrum induced by He-Ne laser is much lower than the former two. Comparisons of those three fluorescence spectra indicate that Ar⁺ laser induced spectrum can show partly fine structure of blood cells. Based on the theoretical analysis, it is presented that the absorption of the fluorophores in blood cells to the wavelength of exciting light has definite selectivity, which depend on energy level structure and state of the fluorophores.

Citation Download Citation

Xiufeng Lan, Shumei Gao, Changde Peng, Ying Liu, and Xiao-Wu Ni "Fluorometric determination of whole blood with various excitation wavelengths", Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); https://doi.org/10.1117/12.546092

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