Investigation on the fluorescent spectral characteristic of ethanol solution induced by different wavelength UV-light

Ying Liu; Tuo Zhu; Xiufeng Lan; Zhonghua Shen; Jian Lu; Xiao-Wu Ni

doi:10.1117/12.546161

8 December 2003 Investigation on the fluorescent spectral characteristic of ethanol solution induced by different wavelength UV-light

Ying Liu, Tuo Zhu, Xiufeng Lan, Zhonghua Shen, Jian Lu, Xiao-Wu Ni

Author Affiliations +

Proceedings Volume 5254, Third International Conference on Photonics and Imaging in Biology and Medicine; (2003) https://doi.org/10.1117/12.546161
Event: Third International Conference on Photonics and Imaging in Biology and Medicine, 2003, Wuhan, China

Abstract

A thorough analysis of the ultraviolet absorption spectra and light induced fluorescence (LIF) spectral characteristics of ethanol solutions is carried out and discussed in theory and in experiment. The results indicate that ethanol solution can absorb UV-light with the wavelength less than 250nm, and can emit very strong fluorescence when excited by these ultraviolet lights. While the wavelengths of excited lights increase, the fluorescent spectral peaks are apparently red-shifted accordingly. As the ethanol solution concentration increases, the fluorescent spectral peaks are also red-shifted. Furthermore, the physical mechanism of absorption and fluorescence emission of ethanol molecule induced by UV light is analyzed based on the theory of molecule orbital structure. The red-shifted reasons of spectral peaks as the excited light wavelength and the solution concentrations changing are also explained. Investigation on the intrinsic fluorescence spectrum of ethanol solution and its characteristics will contribute to the study of the fluorescence spectra when ethanol serves as solute and hydrolysis catalyst.

Citation Download Citation

Ying Liu, Tuo Zhu, Xiufeng Lan, Zhonghua Shen, Jian Lu, and Xiao-Wu Ni "Investigation on the fluorescent spectral characteristic of ethanol solution induced by different wavelength UV-light", Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); https://doi.org/10.1117/12.546161

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