Photobleaching of fluorophores at lower concentration under two-photon excitation microscopy

Tongsheng Chen; Yafeng Liu; Shaoqun Zeng; Qingming Luo

doi:10.1117/12.546559

8 December 2003 Photobleaching of fluorophores at lower concentration under two-photon excitation microscopy

Tongsheng Chen, Yafeng Liu, Shaoqun Zeng, Qingming Luo

Proceedings Volume 5254, Third International Conference on Photonics and Imaging in Biology and Medicine; (2003) https://doi.org/10.1117/12.546559
Event: Third International Conference on Photonics and Imaging in Biology and Medicine, 2003, Wuhan, China

Abstract

The intensity-dependence of photobleaching rate with two-photon excitation (TPE) at biological imaging level has been found to be high-order (>3) relationship. This paper studied experimentally the dependence for rhodamine B (RhB) at two different concentrations under unsaturated absorption conditions. The log-log plot of photobleaching rate versus incident power at concentration of 10^-2M increased with a slope of ~3.7, but that at concentration of 10^-4M increased with a slope of ~2.7.

Citation Download Citation

Tongsheng Chen, Yafeng Liu, Shaoqun Zeng, and Qingming Luo "Photobleaching of fluorophores at lower concentration under two-photon excitation microscopy", Proc. SPIE 5254, Third International Conference on Photonics and Imaging in Biology and Medicine, (8 December 2003); https://doi.org/10.1117/12.546559

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