In vitro biochemical monitoring with fiber-optic-based surface plasmon resonance sensors

Jean-Francois Masson; Kayla Hamersky; Stephen Beaudoin; Karl S. Booksh

doi:10.1117/12.517798

15 March 2004 In vitro biochemical monitoring with fiber-optic-based surface plasmon resonance sensors

Jean-Francois Masson, Kayla Hamersky, Stephen Beaudoin, Karl S. Booksh

Proceedings Volume 5261, Smart Medical and Biomedical Sensor Technology; (2004) https://doi.org/10.1117/12.517798
Event: Optical Technologies for Industrial, Environmental, and Biological Sensing, 2003, Providence, RI, United States

Abstract

An in vitro sensor to detect cardiac markers at less than 2ng/mL and in less than 5 minutes has been achieved. This fiber optic based Surface Plasmon Resonance (SPR) sensor is being applied to detect myoglobin and cardiac Troponin I. Early detection of the onset of myocardial infarction (MI) will greatly enhance the patient care. Myoglobin (MG) and cardiac Troponin I (cTnI) are two biological markers released after a MI. The detection at biologically relevant levels can be diagnostic of MI. Antibodies specific to the antigen of interest are attached to a carboxymethylated dextran (CM-dextran) gold SPR surface. With the method developed, the detection limit for MG is lower than 5ng/mL when detected at 25°C. For cTnI, a detection limit of less than 2ng/mL was achieved in the preliminary tests. For MG, the reaction conditions for antibody attachment of pH 5-6 and a temperature of 37°C gave the highest sensitivity to MG. At pH 6 and 37°C reaction conditions for the antibody attachment were optimal for cTnI. The CM-dextran chain length also influences the antibody loading on the surface: longer CM-dextran chain gives a higher sensitivity to cTnI. The sensor fouls when in contact with serum. Replacing CM-dextran with different biocompatible polymers greatly improve the sensor performance in serum.

Citation Download Citation

Jean-Francois Masson, Kayla Hamersky, Stephen Beaudoin, and Karl S. Booksh "In vitro biochemical monitoring with fiber-optic-based surface plasmon resonance sensors", Proc. SPIE 5261, Smart Medical and Biomedical Sensor Technology, (15 March 2004); https://doi.org/10.1117/12.517798

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