Laser background characterization in a monolithically integrated bio-fluorescence sensor

Evan P. Thrush; Ofer Levi; Laura J. Cook; Jason Deich; Stephen J. Smith; W. E. Moerner; James S. Harris Jr.

doi:10.1117/12.525133

1 July 2004 Laser background characterization in a monolithically integrated bio-fluorescence sensor

Evan P. Thrush, Ofer Levi, Laura J. Cook, Jason Deich, Stephen J. Smith, W. E. Moerner, James S. Harris Jr.

Proceedings Volume 5318, Advanced Biomedical and Clinical Diagnostic Systems II; (2004) https://doi.org/10.1117/12.525133
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Miniaturized and robust sensing modules are required for the development of portable integrated biological analysis systems or micro total analysis systems (μ-TAS). This work uses vertical cavity surface emitting lasers (VCSELs), optical emission filters and PIN photodetectors to realize a monolithically integrated, near-infrared, fluorescence detection system. The integration of these optoelectronic devices with biochips will drastically reduce cost of current systems and increase parallelism and portability. The sensor has been implemented on a micro-fluidic format, and sensitivity was evaluated. A theoretical limit of detection of IR-800 dye in methanol is reported to be 40 nM. The sensor sensitivity is limited by laser background as a result of integrating the optoelectronic elements in such close proximity. Significant reduction in laser background from reflections above the sensor is possible by increasing the distance between the sensor and optical interfaces to greater than 3 mm. Also, for distances greater than 500 microns between the sensor and optical interfaces above the sensor, it is found that background from indirect spontaneous emission is much smaller than the background caused by specular reflections of the laser.

Citation Download Citation

Evan P. Thrush, Ofer Levi, Laura J. Cook, Jason Deich, Stephen J. Smith, W. E. Moerner, and James S. Harris Jr. "Laser background characterization in a monolithically integrated bio-fluorescence sensor", Proc. SPIE 5318, Advanced Biomedical and Clinical Diagnostic Systems II, (1 July 2004); https://doi.org/10.1117/12.525133

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