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1 July 2004 Topographic mapping of biological specimens: flexure and curvature characterization
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Shape quantification of tissue and biomaterials can be central to many studies and applications in bioengineering and biomechanics. Often, shape is mapped with photogrammetry or projected light techniques that provide XYZ point cloud data, and shape is quantified using derived flexure and curvature calculations based on the point cloud data. Accordingly, the accuracy of the calculated curvature depends on the properties of the point cloud data set. In this study, we present a curvature variability prediction (CVP) software model that predicts the distribution, i.e., the standard deviation, of curvature measurements associated with surface topography point cloud data properties. The CVP model point cloud data input variables include XYZ noise, sampling density, and map extent. The CVP model outputs the curvature variability statistic in order to assess performance in the curvature domain. Representative point cloud data properties are obtained from an automated biological specimen video topographer, the BioSpecVT (ver. 1.02) (Vision Metrics, Inc.,). The BioSpecVT uses a calibrated, structured light pattern to support automated computer vision feature extraction software for precisely converting video images of biological specimens, within seconds, into three dimensional point cloud data. In representative sample point cloud data obtained with the BioSpecVT, sampling density is about 11 pts/mm2 for an XYZ mapping volume encompassing about 16 mm x 13.5 mm x 18.5 mm, average XY per point variability is about ±2 μm, and Z axis variability is about ±40 μm (50% level) with a Gaussian distribution. A theoretical study with the CVP model shows that for derived point cloud data properties, curvature mapping accuracy increases, i.e. measurement variability decreases, when curvature increases from about 30 m-1 to 137 m-1. This computed result is consistent with the Z axis noise becoming less significant as the measured depth increases across an approximately fixed XY region.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
William S. Baron and Sandra F. Baron "Topographic mapping of biological specimens: flexure and curvature characterization", Proc. SPIE 5319, Laser Interaction with Tissue and Cells XV, (1 July 2004);


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