Thermoacoustic in vivo determination of blood oxygenation

William L. Kiser Jr.; Robert A. Kruger; Daniel R. Reinecke; Gabe A. Kruger; Kathy D. Miller

doi:10.1117/12.532666

12 July 2004 Thermoacoustic in vivo determination of blood oxygenation

William L. Kiser Jr., Robert A. Kruger, Daniel R. Reinecke, Gabe A. Kruger, Kathy D. Miller

Proceedings Volume 5320, Photons Plus Ultrasound: Imaging and Sensing; (2004) https://doi.org/10.1117/12.532666
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We have utilized a prototype Thermoacoustic Computed Tomography Small Animal Imaging System to acquire images of athymic mice with bilateral tumors implanted in the cranial mammary fat pads. The breast tumor cell lines used in the study, which are MCF7, and MCF7 transfected with Vascular Endothelial Growth Factor (VEGF), exhibit distinctly contrasting levels of vascularization. Three dimensional images of the mice, acquired using pulses of NIR stimulating light, demonstrate the ability of the system to generate high resolution images of the vascular system up to one inch deep in tissue, and at the same time, differentiate tissue types based on the infrared absorption properties of the tissue; a property related in part to blood content and oxygenation levels. We have processed images acquired at different stimulating wavelengths to generate images representative of the distribution of oxygenated and deoxygenated hemoglobin throughout the tumors. The images demonstrate the in vivo capabilities of the imaging system and map system structure as well as the total, oxygenated and deoxygenated hemoglobin components of the blood.

Citation Download Citation

William L. Kiser Jr., Robert A. Kruger, Daniel R. Reinecke, Gabe A. Kruger, and Kathy D. Miller "Thermoacoustic in vivo determination of blood oxygenation", Proc. SPIE 5320, Photons Plus Ultrasound: Imaging and Sensing, (12 July 2004); https://doi.org/10.1117/12.532666

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