DNA hybridization using surface plasmon-coupled emission

Joanna Malicka; Ignacy Gryczynski; Zygmunt Gryczynski; Joseph R. Lakowicz

doi:10.1117/12.530001

1 July 2004 DNA hybridization using surface plasmon-coupled emission

Joanna Malicka, Ignacy Gryczynski, Zygmunt Gryczynski, Joseph R. Lakowicz

Proceedings Volume 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies; (2004) https://doi.org/10.1117/12.530001
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We described a new approach to measuring DNA hybridization using surface plasmon-coupled emission (SPCE). This phenomenon occurs for fluorophores within few hundreds of nanometers of a thin metal film on a glass substrate, in our case a 50 nm thick silver film. Excited fluorophores coupled with the surface plasmons in the metal resulting in directional emission through the glass substrate. We studied the emission of Cy3-labeled DNA oligomers bound to complementary unlabeled biotinylated-oligomers, which were bound to the metal surface via a streptavidin-BSA monolayer. Hybridization resulted in directional emission of Cy3-DNA into the prism. Additionally, the use of SPCE resulted in suppression of interfering emission from non-complementary Cy5-DNA oligomers due to weaker coupling of the more distant fluorophores with the surface plasmons. A large fraction of the total potential emission can couple to the surface plasmon resulting in improved sensitivity. We expect SPCE to have numerous applications to nucleic acid analyses.

Citation Download Citation

Joanna Malicka, Ignacy Gryczynski, Zygmunt Gryczynski, and Joseph R. Lakowicz "DNA hybridization using surface plasmon-coupled emission", Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, (1 July 2004); https://doi.org/10.1117/12.530001

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