Fluorescence lifetime imaging with a multifocal two-photon microscope

Sandrine Leveque-Fort; Marie-Pierre Fontaine-Aupart; Gerard Roger; Patrick Georges

doi:10.1117/12.529912

21 June 2004 Fluorescence lifetime imaging with a multifocal two-photon microscope

Sandrine Leveque-Fort, Marie-Pierre Fontaine-Aupart, Gerard Roger, Patrick Georges

Proceedings Volume 5323, Multiphoton Microscopy in the Biomedical Sciences IV; (2004) https://doi.org/10.1117/12.529912
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Two photon microscopy is a powerful tool for cells or tissues imaging. However it presents the drawback of being a laser-scanning technique leading to long acquisition time for fluorescence lifetime imaging. Thus it is commonly limited to intensity images that only give indications on location of fluorophores but hardly reports physico-chemical properties and interactions between cells components. To preserve biological samples from too long experiments and provide a more complete spectroscopic tool we developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition while keeping the possibility to measure both intensity and lifetime images for all multifocal points.

Citation Download Citation

Sandrine Leveque-Fort, Marie-Pierre Fontaine-Aupart, Gerard Roger, and Patrick Georges "Fluorescence lifetime imaging with a multifocal two-photon microscope", Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, (21 June 2004); https://doi.org/10.1117/12.529912

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