Quantification of bronchial circulation perfusion in rats

Christian Wietholt; Robert C. Molthen; Steven T. Haworth; David L. Roerig; Christopher A. Dawson; Anne V. Clough

doi:10.1117/12.536814

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30 April 2004 Quantification of bronchial circulation perfusion in rats

Christian Wietholt, Robert C. Molthen, Steven T. Haworth, David L. Roerig, Christopher A. Dawson, Anne V. Clough

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Proceedings Volume 5369, Medical Imaging 2004: Physiology, Function, and Structure from Medical Images; (2004) https://doi.org/10.1117/12.536814
Event: Medical Imaging 2004, 2004, San Diego, California, United States

Abstract

The bronchial circulation is thought to be the primary blood supply for pulmonary carcinomas. Thus, we have developed a method for imaging and quantifying changes in perfusion in the rat lung due to development of the bronchial circulation. A dual-modality micro-CT/SPECT system was used to detect change in perfusion in two groups of rats: controls and those with a surgically occluded left pulmonary artery. Both groups were imaged following injections on separate days i) 2mCi of Tc99m labeled macroaggregated albumin (MAA) into the left carotid artery (IA) and ii) a similar injection into the femoral vein (IV). The IA injection resulted in Tc99m accumulation in capillaries of the systemic circulation including the bronchial circulation, whereas the IV resulted in Tc99m accumulation in the pulmonary capillaries. Ordered subset expectation maximization (OSEM) was used to reconstruct the SPECT image volumes and a Feldkamp algorithm was used to reconstruct the micro-CT image volumes. The micro-CT and SPECT volumes were registered, the SPECT image volume was segmented using the right and left lung boundaries defined from the micro-CT volume, and the ratio of IA radioactivity accumulation in the left lung to IV radioactivity accumulation in both lungs was used as a measure of left lung flow via the bronchial circulation. This ratio was ~0.02 for the untreated rats compared to the treated animals that had an increased flow ratio of ~0.21 40 days after left pulmonary artery occlusion. This increase in flow to the occluded left lung via the bronchial circulation suggests this will be a useful model for further investigating antiangiogenic treatments.

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Christian Wietholt, Robert C. Molthen, Steven T. Haworth, David L. Roerig, Christopher A. Dawson, and Anne V. Clough "Quantification of bronchial circulation perfusion in rats", Proc. SPIE 5369, Medical Imaging 2004: Physiology, Function, and Structure from Medical Images, (30 April 2004); https://doi.org/10.1117/12.536814

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