Reagentless cell lysis on a PDMS CD using beads

Jitae Kim; She-Hee Jang; Jim V. Zoval; Nancy A. Da Silva; Marc J. Madou

doi:10.1117/12.548757

16 August 2004 Reagentless cell lysis on a PDMS CD using beads

Jitae Kim, She-Hee Jang, Jim V. Zoval, Nancy A. Da Silva, Marc J. Madou

Proceedings Volume 5455, MEMS, MOEMS, and Micromachining; (2004) https://doi.org/10.1117/12.548757
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

Reagentless mechanical cell lysis was demonstrated on a microfluidic CD (Compact Disc) microfabricated in PDMS (Polydimethylsiloxane). The motion of beads in a lysis chamber on the CD causes disruption of mammalian (CHO-K1), bacterial (Escherichia coli), and yeast (Saccharomyces cerevisiae) cells. Interactions between beads and cells are generated in the rimming flow established inside a partially filled annular chamber in the CD rotating around a horizontal axis. To maximize bead-cell interactions, the CD was spun forward and backwards around this axis, using high acceleration for up to 7 minutes. Based on our theoretical work, we investigated the following control parameters: bead density, angular velocity, acceleration rate, and solid volume fraction, all of which influence cell lysis efficiency. Cell disruption efficiency was verified either through direct microscopic viewing or measurement of DNA concentration after cell lysing. Lysis efficiency relative to a conventional lysis protocol was also determined. In the long term, this work is geared towards CD based sample-to-answer nucleic acid analysis which will include cell lysis, DNA purification, DNA amplification, and DNA hybridization detection.

Citation Download Citation

Jitae Kim, She-Hee Jang, Jim V. Zoval, Nancy A. Da Silva, and Marc J. Madou "Reagentless cell lysis on a PDMS CD using beads", Proc. SPIE 5455, MEMS, MOEMS, and Micromachining, (16 August 2004); https://doi.org/10.1117/12.548757

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