Translator Disclaimer
Paper
10 September 2004 The point spread function of fluorescence microscopes imaging through a layered or an inhomogeneous medium
Author Affiliations +
Abstract
The optical microscope has proven to be an invaluable tool in biology, because of its unique capabilities of 3-D imaging of living specimens. However, compared to other techniques, the achievable resolution is limited. Several techniques have been proposed to improve the resolution of the fluorescence microscope. The confocal set-up is the first of them. Interference effects can also be used to sharpen up the point spread function (PSF) in 4Pi microscopy. Another approach is the so-called STimulated Emission Depletion microscopy, which has permitted to decrease the resolution down to about 100 nm in three dimensions, and below 50 nm in either the x-y plane, or along the z-axis.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Olivier Haeberle and Bertrand Simon "The point spread function of fluorescence microscopes imaging through a layered or an inhomogeneous medium", Proc. SPIE 5462, Biophotonics Micro- and Nano-Imaging, (10 September 2004); https://doi.org/10.1117/12.545792
PROCEEDINGS
10 PAGES


SHARE
Advertisement
Advertisement
Back to Top