Automatic enhancement of skin fluorescence localization due to refractive index matching

Dmitry Y. Churmakov; Igor Vladislavovich Meglinski; Sergey A. Piletsky; Douglas A. Greenhalgh

doi:10.1117/12.571358

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29 July 2004 Automatic enhancement of skin fluorescence localization due to refractive index matching

Dmitry Y. Churmakov, Igor Vladislavovich Meglinski, Sergey A. Piletsky, Douglas A. Greenhalgh

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Proceedings Volume 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics; (2004) https://doi.org/10.1117/12.571358
Event: ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, 2003, Silsoe, United Kingdom

Abstract

Fluorescence diagnostic techniques are notable amongst many other optical methods, as they offer high sensitivity and non-invasive measurements of tissue properties. However, a combination of multiple scattering and physical heterogeneity of biological tissues hampers the interpretation of the fluorescence measurements. The analyses of the spatial distribution of endogenous and exogenous fluorophores excitations within tissues and their contribution to the detected signal localization are essential for many applications. We have developed a novel Monte Carlo technique that gives a graphical perception of how the excitation and fluorescence detected signal are localized in tissues. Our model takes into account spatial distribution of fluorophores and their quantum yields. We demonstrate that matching of the refractive indices of ambient medium and topical skin layer improves spatial localization of the detected fluorescence signal within the tissue. This result is consistent with the recent conclusion that administering biocompatible agents results in higher image contrast.

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Dmitry Y. Churmakov, Igor Vladislavovich Meglinski, Sergey A. Piletsky, and Douglas A. Greenhalgh "Automatic enhancement of skin fluorescence localization due to refractive index matching", Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); https://doi.org/10.1117/12.571358

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