Measuring optical properties of microvolume biopsies

Majid Taraz; Victor B. Loschenov

doi:10.1117/12.572013

29 July 2004 Measuring optical properties of microvolume biopsies

Majid Taraz, Victor B. Loschenov

Proceedings Volume 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics; (2004) https://doi.org/10.1117/12.572013
Event: ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, 2003, Silsoe, United Kingdom

Abstract

Special tissue sampler was developed, allowing measuring concentration of photosensitizer in various biopsy materials with high accuracy. The method is based on simultaneous measurement of fluorescence and reflectance spectra and biopsy of tissue sample embedded in a spherical aperture by a diameter of 1.5 mm and a depth of 1 mm. Both surfaces of the sampler were covered with an impenetrable black film. Meterological measurements were done on standard solutions consisting solutions "Photoscence" and "Intralipid" in various ratios. We used a fiber optic spectrometer LESA-01-BIOSPEC to measure optical properties of the solution with various concentrations of "Intralipid" and "Photoscence." Results follow theory. To measure concentration of photosensitizer in micro volumes and thin layers, special multifiber catheters with external diameter from 0.5 mm to 1.8 mm were prepared.

Citation Download Citation

Majid Taraz and Victor B. Loschenov "Measuring optical properties of microvolume biopsies", Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); https://doi.org/10.1117/12.572013

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