Spectra-resolved technique of a sensitive time-resolved fluorescence immunoassay instrument

Zhouyi Guo; Zhen Tian; Yali Jia

doi:10.1117/12.572442

29 July 2004 Spectra-resolved technique of a sensitive time-resolved fluorescence immunoassay instrument

Zhouyi Guo, Zhen Tian, Yali Jia

Proceedings Volume 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics; (2004) https://doi.org/10.1117/12.572442
Event: ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, 2003, Silsoe, United Kingdom

Abstract

The lanthanide trivalence ion and its chelates are used for marking substance in time-resolved fluorescence immunoassay (TRFIA), marking the protein, hormone, antibody, nucleic acid probe or biologica alive cell, to measure the concentration of the analysis substance inside the reaction system with time-resolved fluorometry after the reaction system occurred, and attain the quantitative analysis's purpose. TRFIA has been become a kind of new and more sensitive measure method after radioisotope marking, enzymatic marking, chemiluminescence, electrochemiluminescence, it primarily is decided by the special physics and chemistry characteristic of lanthanide trivalence ion and its chelates. In this paper, the result of spectroscopic evaluation of europium trivalence ion and its chelate, and the principle of spectra-resolved technology and a sensitive time-resolved fluorescence immunoassay instrument made by ourselves are reported. In the set, a high frequency Xenon pulsed-light was adopted as exciting light, and two special filters was utilized according to spectra-resolved technique. Thus the influence of scattering light and short-lifetime fluorescence was removed. And the sensitivity is 10^-12mol/L (when Eu³⁺ was used for marking substance), examination repeat is CV ≤ 5%, examination linearity is from 10^-8mol/L to 10^-12mol/L, correlation coefficient r ≥ 95% (p < 0.01).

Citation Download Citation

Zhouyi Guo, Zhen Tian, and Yali Jia "Spectra-resolved technique of a sensitive time-resolved fluorescence immunoassay instrument", Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); https://doi.org/10.1117/12.572442

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KEYWORDS

Luminescence

Time resolved spectroscopy

Ions

Lanthanides

Optical filters

Light scattering

Europium

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