Effect of ionic strength on electrically evoked membrane tether force: an optical tweezers study

Feng Qian; William E. Brownell; Bahman Anvari

doi:10.1117/12.560021

18 October 2004 Effect of ionic strength on electrically evoked membrane tether force: an optical tweezers study

Feng Qian, William E. Brownell, Bahman Anvari

Proceedings Volume 5514, Optical Trapping and Optical Micromanipulation; (2004) https://doi.org/10.1117/12.560021
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

We have investigated the effect of ionic strength on electrically evoked membrane tether force using optical tweezers. Membrane tethers from cochlear outer hair cells (OHCs) and human embryonic kidney (HEK) cells exhibited mechanical response to applied voltage stimuli over a wide frequency range. The electrically evoked variations in the tether force were probed by an optically-trapped microsphere, the image of which was projected on a quadrant photodiode for dynamic measurement of its displacements. Compared to normal saline (140mM NaCl), low ionic-strength solution (10mM NaCl) blocked the electrically evoked tether force for both OHCs and HEK cells. As the Debye length for membrane bilayer was estimated to increase from approximately 0.75nm to 1.88nm, the internal repulsive pressure of the membrane tethers rose consequently, resulting in the enlargement in the equilibrium tether diameter and the decrease in the tether force.

Citation Download Citation

Feng Qian, William E. Brownell, and Bahman Anvari "Effect of ionic strength on electrically evoked membrane tether force: an optical tweezers study", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); https://doi.org/10.1117/12.560021

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