Speckle intensity correlation and optical diffusion profile in biological media

Alex Flamholz; Patricia S. Schneider; Peter K. Wong; David H. Lieberman; Tak D. Cheung; Harriet Itoka; Troy Minott; Janie Quizhpi; Jacquelin Rodriguez

doi:10.1117/12.558071

2 August 2004 Speckle intensity correlation and optical diffusion profile in biological media

Alex Flamholz, Patricia S. Schneider, Peter K. Wong, David H. Lieberman, Tak D. Cheung, Harriet Itoka, Troy Minott, Janie Quizhpi, Jacquelin Rodriguez

Author Affiliations +

Proceedings Volume 5531, Interferometry XII: Techniques and Analysis; (2004) https://doi.org/10.1117/12.558071
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

Short-range speckle correlation techniques were used to measure the refractive index of turbid biological media. The refractive index depends on the cell content, which is about 80% water and 15% protein. The variation in water or protein content produced various small shifts in the oscillatory features of the speckle intensity spatial correlation function for correlation distances shorter than the transport mean free path. Optical diffusion profiles in transmission, and long range speckle intensity correlation techniques were used to measure the transport mean free path. The optical system was calibrated with a porous silicate slab, and live yeast was the biological system studied. It is found that the techniques employed could serve as markers for the cell's water and protein contents. Consistent results were also found for chicken tissue and a combined yeast sample. Extension to abnormal cell detection, and the application to in-situ refractive index mapping are also discussed.

Citation Download Citation

Alex Flamholz, Patricia S. Schneider, Peter K. Wong, David H. Lieberman, Tak D. Cheung, Harriet Itoka, Troy Minott, Janie Quizhpi, and Jacquelin Rodriguez "Speckle intensity correlation and optical diffusion profile in biological media", Proc. SPIE 5531, Interferometry XII: Techniques and Analysis, (2 August 2004); https://doi.org/10.1117/12.558071

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