A practical method to determine the light source distribution in bioluminescent imaging

Wenxiang Cong; Durairaj Kumar; Yi Liu; Alexander Cong; Ge Wang

doi:10.1117/12.560524

26 October 2004 A practical method to determine the light source distribution in bioluminescent imaging

Wenxiang Cong, Durairaj Kumar, Yi Liu, Alexander Cong, Ge Wang

Proceedings Volume 5535, Developments in X-Ray Tomography IV; (2004) https://doi.org/10.1117/12.560524
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

Optical signatures of tumor cells may be generated by expression of reporter genes encoding bioluminescent/fluorescent proteins. Bioluminescent imaging is a novel technique that identifies such light sources from the light flux detected on the surface of a small animal. This technique can effectively evaluate tumor cell growth and regression in response to various therapies in medical research, drug development and gene therapy. In this paper, the diffusion approximation is employed to describe the propagation of photons through biological tissues. Then, a practical method is proposed for localizing and quantifying bioluminescent sources from external bioluminescent signals. This method incorporates prior knowledge on permissible source regions, and transforms the inverse bioluminescent problem into a finite element-based constrained optimization procedure. This approach is validated and evaluated with ideal and noise data in numerical simulation.

Citation Download Citation

Wenxiang Cong, Durairaj Kumar, Yi Liu, Alexander Cong, and Ge Wang "A practical method to determine the light source distribution in bioluminescent imaging", Proc. SPIE 5535, Developments in X-Ray Tomography IV, (26 October 2004); https://doi.org/10.1117/12.560524

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