Development of lung tissue phantoms for bioluminescent imaging

Durairaj Kumar; Wenxiang Cong; Frank Bohenkamp; Tarun Kakaday; Peter Taft; Lihong V. Wang; Geoffrey McLennan; Eric A. Hoffman; Ge Wang

doi:10.1117/12.560530

26 October 2004 Development of lung tissue phantoms for bioluminescent imaging

Durairaj Kumar, Wenxiang Cong, Frank Bohenkamp, Tarun Kakaday, Peter Taft, Lihong V. Wang, Geoffrey McLennan, Eric A. Hoffman, Ge Wang

Author Affiliations +

Proceedings Volume 5535, Developments in X-Ray Tomography IV; (2004) https://doi.org/10.1117/12.560530
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

White nylon material was chosen to make cylindrical tissue phantoms for development of bioluminescence tomography techniques. A low-level light source, delivered through an optic fiber of core diameter 200 μm, was placed at different locations on one phantom surface. The light travels through the phantom, reaches the external surface, and is captured by a liquid nitrogen-cooled CCD camera. The scattering, absorption, and anisotropy parameters of the phantom are obtained by matching the measured light transmission profiles to the profiles generated by the TracePro software. The perturbation analysis, with the homogeneous phantoms, demonstrated that the imaging system is sufficiently sensitive to capture intensity change of higher than 0.5nW/cm² or a location shift of the light source of more than 200 microns. It is observed that the system can distinguish two point light sources with separation of about 2 mm. The perturbation analysis is also performed with the heterogeneous phantom. Based on our data, we conclude that there is inherent tomographic information in bioluminescent measures taken on the external surface of the mouse, which suggests the feasibility of bioluminescence tomography for biomedical research using the small animals, especially the mice.

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Durairaj Kumar, Wenxiang Cong, Frank Bohenkamp, Tarun Kakaday, Peter Taft, Lihong V. Wang, Geoffrey McLennan, Eric A. Hoffman, and Ge Wang "Development of lung tissue phantoms for bioluminescent imaging", Proc. SPIE 5535, Developments in X-Ray Tomography IV, (26 October 2004); https://doi.org/10.1117/12.560530

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KEYWORDS

Tissue optics

CCD cameras

Tissues

Tomography

Ocean optics

Imaging systems

Lung

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