Determination of myosin filament positions and orientations in electron micrographs of muscle cross sections

Bjarni Bodvarsson; Soren Klim; Stig Mortensen; Martin Morkebjerg; James Chen; Julian R. Maclaren; Chun Hong Yoon; Pradeep K. Luther; John M. Squire; Andrew Bainbridge-Smith; Philip J. Bones; Rick P. Millane

doi:10.1117/12.562357

22 October 2004 Determination of myosin filament positions and orientations in electron micrographs of muscle cross sections

Bjarni Bodvarsson, Soren Klim, Stig Mortensen, Martin Morkebjerg, James Chen, Julian R. Maclaren, Chun Hong Yoon, Pradeep K. Luther, John M. Squire, Andrew Bainbridge-Smith, Philip J. Bones, Rick P. Millane

Author Affiliations +

Proceedings Volume 5562, Image Reconstruction from Incomplete Data III; (2004) https://doi.org/10.1117/12.562357
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

An automated image analysis system for determination of myosin filament orientations in electron micrographs of muscle cross-sections is described. Analysis of the distribution of the orientations is important in studies of muscle structure, particularly for interpretation of x-ray diffraction data. Filament positions are determined using h-dome extraction and image filtering, based on grayscale reconstruction. Erroneous locations are eliminated based on lattice regularity. Filament orientations are determined by correlation with a template that incorporates the salient filament characteristics and classified using a Gaussian mixture model. Application to a number of micrographs and comparison with manual classifications of orientations shows that the system is effective in many cases.

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Bjarni Bodvarsson, Soren Klim, Stig Mortensen, Martin Morkebjerg, James Chen, Julian R. Maclaren, Chun Hong Yoon, Pradeep K. Luther, John M. Squire, Andrew Bainbridge-Smith, Philip J. Bones, and Rick P. Millane "Determination of myosin filament positions and orientations in electron micrographs of muscle cross sections", Proc. SPIE 5562, Image Reconstruction from Incomplete Data III, (22 October 2004); https://doi.org/10.1117/12.562357

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