Examination of yeast cell parameters using light diffusion

Patricia S. Schneider; Alex Flamholz; Peter K. Wong; David H. Lieberman; Tak D. Cheung; Harriet Itoka; Troy Minott; Janie Quizhpi; Jacquelin Rodriguez

doi:10.1117/12.567406

9 December 2004 Examination of yeast cell parameters using light diffusion

Patricia S. Schneider, Alex Flamholz, Peter K. Wong, David H. Lieberman, Tak D. Cheung, Harriet Itoka, Troy Minott, Janie Quizhpi, Jacquelin Rodriguez

Author Affiliations +

Proceedings Volume 5578, Photonics North 2004: Photonic Applications in Astronomy, Biomedicine, Imaging, Materials Processing, and Education; (2004) https://doi.org/10.1117/12.567406
Event: Photonics North, 2004, Ottawa, Ontario, Canada

Abstract

Cell parameters such as size and density may provide crucial information on issues such as aging and cancer. Measurement of these parameters in bulk, in a cells natural environment are therefore important. For bulk samples light diffusion measurements using transmission spatial profiles and correlation function analysis provide information on the mean free path, absorption and refractive index. Speckle fractal analysis can also provide cell structure information. For cells in suspension forward scattering is used to provide size information via the Mie theory for spherical objects. Two types of live yeast cells were measured in bulk and in suspension at various densities. The bulk samples were compacted by centrifuge into fractions whose mass and volume was measured. The parameter values obtained by optical diffusion were used to infer the density and size variations. The measurement detected density variation of about 10% for yeast grown under normal conditions. The size variation is also about 10% but it contained more uncertainty due to the constant density assumption used in the Mie theory for spherical objects. High resolution optical microscopy confirmed the cell size and showed that it followed a lognormal distribution. The density variation resulted mainly from size differences with a smaller contribution from mass structure such as protein. The results indicate that diffusion measurement is consistent with density measurement and could possibly be used as a cell density probe in clinical applications.

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Patricia S. Schneider, Alex Flamholz, Peter K. Wong, David H. Lieberman, Tak D. Cheung, Harriet Itoka, Troy Minott, Janie Quizhpi, and Jacquelin Rodriguez "Examination of yeast cell parameters using light diffusion", Proc. SPIE 5578, Photonics North 2004: Photonic Applications in Astronomy, Biomedicine, Imaging, Materials Processing, and Education, (9 December 2004); https://doi.org/10.1117/12.567406

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KEYWORDS

Yeast

Speckle

Fractal analysis

Diffusion

Mie scattering

Refractive index

Microscopy

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