Proliferative effect of green light emitting diode irradiation on chicken fibroblasts in hyperglycaemic circumstances: a preliminary <i>in vitro</i> study

Elke Vinck; Barbara Cagnie; Heidi Declercq; Ria Cornelissen; Dirk Cambier

doi:10.1117/12.584395

10 September 2004 Proliferative effect of green light emitting diode irradiation on chicken fibroblasts in hyperglycaemic circumstances: a preliminary in vitro study

Elke Vinck, Barbara Cagnie, Heidi Declercq, Ria Cornelissen, Dirk Cambier

Author Affiliations +

Proceedings Volume 5610, Laser Florence 2003: A Window on the Laser Medicine World; (2004) https://doi.org/10.1117/12.584395
Event: Laser Florence 2003: A Window on the Laser Medicine World, 2003, Florence, Italy

Abstract

A reduced mortality due to hyperglycaemia was noted since the development of insulin treatment for type I diabetes and various oral hypoglycaemic agents for type II diabetes. Nevertheless the chronic metabolic disorder, Diabetes Mellitus, remains an important cause of morbidity and mortality due to a series of common secondary metabolic complications. Patients with diabetes have an increased tendency to develop infections of the skin. Healing of skin lesions in diabetics evolves often relatively slow and the lesions tend to be more severe than in non-diabetics. Endeavouring to accelerate the healing process of skin lesions in diabetic patients, this preliminary in vitro study investigates the efficacy of green Light Emitting Diode (LED) irradiation on fibroblast proliferation of cells in hyperglycaemic circumstances. In an attempt to imitate the diabetic environment, embryonic chicken fibroblasts were cultured in hyperglycaemic medium (30.000mg Glucose per litre Hanks Medium). LED irradiation was performed three consecutive days with a wavelength of 540 nm and a power output of 10 mW, at 0,6 cm distance from the fibroblasts. Each treatment lasted 3 minutes, resulting in a surface energy density of 0,2 J/cm². Statistical analysis revealed that LED irradiation at the applied parameters induced a higher rate of proliferation in hyperglycaemic circumstances after irradiation than in the same circumstances without irradiation. Regarding these results the effectiveness of green LED irradiation on cells in hyperglycaemic circumstances is proven. To ensure the effectiveness and to evaluate the value of LED irradiation in vivo, further research is required.

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Elke Vinck, Barbara Cagnie, Heidi Declercq, Ria Cornelissen, and Dirk Cambier "Proliferative effect of green light emitting diode irradiation on chicken fibroblasts in hyperglycaemic circumstances: a preliminary in vitro study", Proc. SPIE 5610, Laser Florence 2003: A Window on the Laser Medicine World, (10 September 2004); https://doi.org/10.1117/12.584395

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KEYWORDS

Glucose

Light emitting diodes

In vitro testing

In vivo imaging

Skin

Green light emitting diodes

Wound healing

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