Detection of immune reaction by dynamic enhanced vibrational spectroscopy of molecules in near field

Haiying Zhao; Xiaoming Dou

doi:10.1117/12.575753

18 January 2005 Detection of immune reaction by dynamic enhanced vibrational spectroscopy of molecules in near field

Haiying Zhao, Xiaoming Dou

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.575753
Event: Photonics Asia, 2004, Beijing, China

Abstract

This paper reports the near-infrared (NIR) SERS detection of immune reaction on gold colloid particles without bound/Free (B/F) antigen separation. Antibodies or the immune complex are adsorbed on gold colloid particles. Free antigen cannot be adsorbed on the gold colloid particles due to the surface of the gold colloid particles is blocked by bovine serum albumin. Because of the electromagnetic field generated by surface plasmon polariton enhancement, the antibody at 10^-8 M on the gold colloid particles shows intense SERS signals. The same system at 10^-10 M does not give any SERS signal, but Raman bands of antibody again appear upon the formation of immunoglobulin (IgG)-anti-IgG complexes on the gold colloid particles. Free and bound antigen molecules do not yield significant SERS signals since they cannot be adsorbed directly on the gold surface. In this way, one could detect the immune complex on the gold colloid particles by NIR SERS spectroscopy without any B/F antigen separation.

Citation Download Citation

Haiying Zhao and Xiaoming Dou "Detection of immune reaction by dynamic enhanced vibrational spectroscopy of molecules in near field", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.575753

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