Electrochemiluminescence-PCR detection of genetically modified organisms

Jinfeng Liu; Da Xing; Xingyan Shen; Debin Zhu

doi:10.1117/12.572808

18 January 2005 Electrochemiluminescence-PCR detection of genetically modified organisms

Jinfeng Liu, Da Xing, Xingyan Shen, Debin Zhu

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.572808
Event: Photonics Asia, 2004, Beijing, China

Abstract

The detection methods for genetically modified (GM) components in foods have been developed recently. But many of them are complicated and time-consuming; some of them need to use the carcinogenic substance, and can’t avoid false-positive results. In this study, an electrochemiluminescence polymerase chain reaction (ECL-PCR) method for detection GM tobaccos is proposed. The Cauliflower mosaic virus 35S (CaMV35S) promoter was amplified by PCR, Then hybridized with a Ru(bpy)32+ (TBR)-labeled and a biotinylated probe. The hybridization products were captured onto streptavidin-coated paramagnetic beads, and detected by measuring the electrochemiluminescence (ECL) signal of the TBR label. Whether the tobaccos contain GM components was discriminated by detecting the ECL signal of CaMV35S promoter. The experiment results show that the detection limit for CaMV35S promoter is 100 fmol, and the GM components can be clearly identified in GM tobaccos. The ECL-PCR method provide a new means in GMOs detection due to its safety, simplicity and high efficiency.

Citation Download Citation

Jinfeng Liu, Da Xing, Xingyan Shen, and Debin Zhu "Electrochemiluminescence-PCR detection of genetically modified organisms", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.572808

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