Molecular beacon-based photodynamic therapy

Juan Chen; Klara Stefflova; Soungkyoo Kim; Hui Li; Diane Marotta; Britton Chance; Jerry D. Glickson; Gang Zheng

doi:10.1117/12.573915

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18 January 2005 Molecular beacon-based photodynamic therapy

Juan Chen, Klara Stefflova, Soungkyoo Kim, Hui Li, Diane Marotta, Britton Chance, Jerry D. Glickson, Gang Zheng

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Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.573915
Event: Photonics Asia, 2004, Beijing, China

Abstract

A new concept for photodynamic therapy (PDT) has been developed based on incorporating a photosensitizer (PS) and a singlet oxygen (¹O₂) quenching/scavenging molecule (Q) onto a disease-targeting carrier, such that the PS becomes activatable by light only when targeting has occurred. This has the potential to give very high disease specificity in PDT treatment. The first model compound designed using this concept was synthesized containing a pyropheophorbide as the PS and a carotenoid as the ¹O₂ quencher. These were kept in close proximity by the self-folding of a caspase-3 specific peptide sequence. Upon caspase-3-induced cleavage, the ¹O₂ production increase has been validated by direct ¹O₂ luminescence and lifetime measurements, providing proof-of-concept of this 'PDT beacon.'

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Juan Chen, Klara Stefflova, Soungkyoo Kim, Hui Li, Diane Marotta, Britton Chance, Jerry D. Glickson, and Gang Zheng "Molecular beacon-based photodynamic therapy", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.573915

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