AFM analysis of the formation of DNA aggregates on polymeric biochips

Prashant D. Sawant; Gregory Watson; Sverre Myhra; Dan V. Nicolau

doi:10.1117/12.584142

16 February 2005 AFM analysis of the formation of DNA aggregates on polymeric biochips

Prashant D. Sawant, Gregory Watson, Sverre Myhra, Dan V. Nicolau

Proceedings Volume 5651, Biomedical Applications of Micro- and Nanoengineering II; (2005) https://doi.org/10.1117/12.584142
Event: Smart Materials, Nano-, and Micro-Smart Systems, 2004, Sydney, Australia

Abstract

The demand for polymer-based DNA microarrays will increase because of their cost-effectiveness, biocompatibility and easy processing. However not all polymers are ideal substrates because of different chemical interactions of polymeric substrates with the DNA molecules. Results from AFM analysis of DNA immobilised on polymeric surfaces are evaluated using fractality, Gaussian distribution and lateral force imaging. It has been found that the nanosize defects in the substrate, such as poly-l-lysine, plays an important role in the growth of DNA aggregates in a vertical direction, whereas the covalent binding of DNA molecules on NHS-functionalised cyclo-olefin copolymer leads to the lateral growth of DNA aggregates.

Citation Download Citation

Prashant D. Sawant, Gregory Watson, Sverre Myhra, and Dan V. Nicolau "AFM analysis of the formation of DNA aggregates on polymeric biochips", Proc. SPIE 5651, Biomedical Applications of Micro- and Nanoengineering II, (16 February 2005); https://doi.org/10.1117/12.584142

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