Polymer microstructures for cellular growth studies

Luisa Filipponi; Kristi L. Hanson; Abraham Phillip Lee; Dan V. Nicolau

doi:10.1117/12.584143

16 February 2005 Polymer microstructures for cellular growth studies

Luisa Filipponi, Kristi L. Hanson, Abraham Phillip Lee, Dan V. Nicolau

Proceedings Volume 5651, Biomedical Applications of Micro- and Nanoengineering II; (2005) https://doi.org/10.1117/12.584143
Event: Smart Materials, Nano-, and Micro-Smart Systems, 2004, Sydney, Australia

Abstract

The understanding and control of cell growth in confined microenvironments has application to a variety of fields including cell biosensor development, medical device fabrication, and pathogen control. While the majority of work in these areas has focused on mammalian and bacterial cell growth, this study reports on the growth behavior of fungal cells in three-dimensionally PDMS microenvironments of a scale similar to that of individual hyphae. Confinement was found to affect filament branching rate and angle. Overall, fungal hyphae demonstrate much more coordinated behavior during confinement than observed during growth on simple planar unconfined substrates. The remarkable difference of fungal growth behaviour observed in the PDMS microenvironments compared to open, unrestricted environments suggests that three-dimensional microstructures could be used to control and alter fungal motility.

Citation Download Citation

Luisa Filipponi, Kristi L. Hanson, Abraham Phillip Lee, and Dan V. Nicolau "Polymer microstructures for cellular growth studies", Proc. SPIE 5651, Biomedical Applications of Micro- and Nanoengineering II, (16 February 2005); https://doi.org/10.1117/12.584143

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available