Novel approach to process protein crystals using deep-UV laser

Hiroshi Kitano; Atsushi Sato; Atsutoshi Murakami; Hiroaki Adachi; Hiroyoshi Matsumura; Kazufumi Takano; Tsuyoshi Inoue; Yusuke Mori; Masaaki Doi; Takatomo Sasaki

doi:10.1117/12.596331

8 October 2004 Novel approach to process protein crystals using deep-UV laser

Hiroshi Kitano, Atsushi Sato, Atsutoshi Murakami, Hiroaki Adachi, Hiroyoshi Matsumura, Kazufumi Takano, Tsuyoshi Inoue, Yusuke Mori, Masaaki Doi, Takatomo Sasaki

Author Affiliations +

Proceedings Volume 5662, Fifth International Symposium on Laser Precision Microfabrication; (2004) https://doi.org/10.1117/12.596331
Event: Fifth International Symposium on Laser Precision Microfabrication, 2004, Nara, Japan

Abstract

We propose new application of a pulsed deep-UV laser to processing biological macromolecule crystals. Single crystals of model protein, hen egg white lysozyme, were processed by deep-UV laser irradiation at 193 nm and 266 nm. Desired laser ablation was achieved using the 193-nm light source. A crystallinity of the processed crystals was evaluated by X-ray diffraction pattern recording. The crystal diffracted beyond a 1.9 Å resolution, which was the same as that of the as-grown crystal obtained under identical growth conditions. In addition, we observed that a diffraction pattern from a cracked protein crystal was considerably improved by eliminating the damaged section with the 193-nm laser ablation. A processing tool for protein crystals currently employed, such as a micro needle or knife, suffers from low reproducibility and poor accuracy. The technique using deep-UV laser pulses will be a powerful tool for processing very fragile protein crystals.

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Hiroshi Kitano, Atsushi Sato, Atsutoshi Murakami, Hiroaki Adachi, Hiroyoshi Matsumura, Kazufumi Takano, Tsuyoshi Inoue, Yusuke Mori, Masaaki Doi, and Takatomo Sasaki "Novel approach to process protein crystals using deep-UV laser", Proc. SPIE 5662, Fifth International Symposium on Laser Precision Microfabrication, (8 October 2004); https://doi.org/10.1117/12.596331

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