Ex-vivo crystalline lens optical quality measurements during simulated accommodation using a single pass PSF measurement system

David Borja; Gaku Takeuchi; Katsuhiko Kobayashi; Fabrice Manns; Jean Marie Parel

doi:10.1117/12.600618

18 April 2005 Ex-vivo crystalline lens optical quality measurements during simulated accommodation using a single pass PSF measurement system

David Borja, Gaku Takeuchi, Katsuhiko Kobayashi, Fabrice Manns, Jean Marie Parel

Author Affiliations +

Proceedings Volume 5688, Ophthalmic Technologies XV; (2005) https://doi.org/10.1117/12.600618
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Purpose: To design and test an optical system to measure the optical quality of post mortem lenses during simulated accommodation. Methods: An optical bench top system was designed to measure the point spread function and calculate the modulation transfer function (MTF) of monkey and human ex-vivo crystalline lenses. The system consists of a super luminescent diode emitting at 850nm, collimated into a 3mm beam which is focused by the ex-vivo lens under test. The intensity distribution at the focus (point spread function) is re-imaged and magnified onto a beam profiler CCD camera. The optical quality in terms of spatial frequency response (modulation transfer function) is calculated by Fourier transform of the point spread function. The system was used on ex-vivo lenses with attached zonules, ciliary body and sclera. The sclera was glued to 8 separate PMMA segments and stretched radial by 5mm on an accommodation simulating lens stretching device. The point spread function was measured for each lens in the relaxed and stretched state for 5 human (ages 38-86 years) and 5 cynomolgus monkey (ages 53 - 67 months) fresh post mortem crystalline lenses. Results: Stretching induced measurable changes in the MTF. The cutoff frequency increased from 54.4±13.6 lp/mm unstretched to 59.5±21.4 lp/mm stretched in the post-presbyopic human and from 51.9±24.7 lp/mm unstretched to 57.7±18.5 lp/mm stretched cynomolgus monkey lenses. Conclusion: The results demonstrate the feasibility of measuring the optical quality of ex-vivo human and cynomolgus monkey lenses during simulated accommodation. Additional experiments are underway to quantify changes in optical quality induced by stretching.

Citation Download Citation

David Borja, Gaku Takeuchi, Katsuhiko Kobayashi, Fabrice Manns, and Jean Marie Parel "Ex-vivo crystalline lens optical quality measurements during simulated accommodation using a single pass PSF measurement system", Proc. SPIE 5688, Ophthalmic Technologies XV, (18 April 2005); https://doi.org/10.1117/12.600618

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