Instrumentation for the measurement of autofluorescence in human skin

Reindert Graaff; Robbert Meerwaldt M.D.; Helen L Lutgers M.D.; Rene Baptist; Ed D de Jong; Jaap R Zijp; Thera P Links M.D.; Andries J Smit M.D.; Gerhard Rakhorst

doi:10.1117/12.588984

1 April 2005 Instrumentation for the measurement of autofluorescence in human skin

Reindert Graaff, Robbert Meerwaldt M.D., Helen L Lutgers M.D., Rene Baptist, Ed D de Jong, Jaap R Zijp, Thera P Links M.D., Andries J Smit M.D., Gerhard Rakhorst

Author Affiliations +

Proceedings Volume 5692, Advanced Biomedical and Clinical Diagnostic Systems III; (2005) https://doi.org/10.1117/12.588984
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

A setup to measure skin autofluorescence was developed to assess accumulation of advanced glycation endproducts (AGE) in patients noninvasively. The method applies direct blacklight tube illumination of the skin of the lower arm, and spectrometry. The setup displays skin autofluorescence (AF) as a ratio of mean intensities detected from the skin between 420-600 nm and 300-420 nm, respectively. In an early clinical application in 46 and control subjects matched for age and gender, AF was significantly increased in the patients (p = 0.015), and highly correlated with skin AGE's that were determined from skin biopsies in both groups. A large follow-up study on type 2 diabetes mellitus, ongoing since 2001 with more than 1000 subjects, aims to assess the value of the instrument in predicting chronic complications of diabetes. At baseline, a relation with age, glycemic status and with complications present was found. In a study in patients with end stage renal disease on dialysis AF was a strong and independent predictor of total and cardiovascular mortality. A commercial version of this AGE-reader is now under development and becomes available early 2005 (DiagnOptics B.V., Groningen, The Netherlands). One of the remaining questions, that will be answered by measuring so-called Exciation-Emission Matrices (EEM's) of the skin tissue in vivo, is whether a more selective choice of wavelengths is more strongly related to clinical characteristics. An experimental instrument to measure these EEM's was, therefore, developed as well. Clinical measurements are underway of EEM's in patient groups with diabetes mellitus and in healthy volunteers.

Citation Download Citation

Reindert Graaff, Robbert Meerwaldt M.D., Helen L Lutgers M.D., Rene Baptist, Ed D de Jong, Jaap R Zijp, Thera P Links M.D., Andries J Smit M.D., and Gerhard Rakhorst "Instrumentation for the measurement of autofluorescence in human skin", Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); https://doi.org/10.1117/12.588984

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available