Surface-enhanced Raman scattering molecular nanoprobes

Musundi B. Wabuyele; Fei Yan; Guy D Griffin; Tuan Vo-Dinh

doi:10.1117/12.604447

1 April 2005 Surface-enhanced Raman scattering molecular nanoprobes

Musundi B. Wabuyele, Fei Yan, Guy D Griffin, Tuan Vo-Dinh

Proceedings Volume 5692, Advanced Biomedical and Clinical Diagnostic Systems III; (2005) https://doi.org/10.1117/12.604447
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Nanoparticles are increasingly finding a wide application in the biological studies due to their unique physical and chemical properties. Colloidal nanoparticles are efficient substrate that exhibit surface-enhanced Raman scattering (SERS) phenomenon by enhancing the scattering cross-sections of conjugated Raman active molecules thus enabling highly sensitive biological probes. However, biological and medical applications would require nanoparticles to be conjugated to biomolecules. A universal approach for conjugation of mercarptoacetic acid-capped silver nanoparticles to biomolecules is described. The surface functionalized silver colloids were labeled with a Raman active dye and used for cellular imaging. We also described the use of silver nanoparticle to develop a new class of SERS nanoprobes for molecular recognition and detection of specific nucleic acid sequences.

Citation Download Citation

Musundi B. Wabuyele, Fei Yan, Guy D Griffin, and Tuan Vo-Dinh "Surface-enhanced Raman scattering molecular nanoprobes", Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); https://doi.org/10.1117/12.604447

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