FT-IR analysis of phosphorylation and laser-dephosphorylation process

Katsunori Ishii; Kaoru Yanase; Sachiko Suzuki-Yoshihashi; Masaya Yamamoto; Kunihiro Chihara; Yasuhiko Tabata; Kunio Awazu

doi:10.1117/12.591904

15 April 2005 FT-IR analysis of phosphorylation and laser-dephosphorylation process

Katsunori Ishii, Kaoru Yanase, Sachiko Suzuki-Yoshihashi, Masaya Yamamoto, Kunihiro Chihara, Yasuhiko Tabata, Kunio Awazu

Author Affiliations +

Proceedings Volume 5695, Optical Interactions with Tissue and Cells XVI; (2005) https://doi.org/10.1117/12.591904
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Phosphorylation and dephosphorylation, which are the most remarkable posttranslational modifications, are considered to be important chemical reactions that control the activation of proteins. In regenerative medicine, it has been reported that phosphorylated proteins are instrumental in the calcification of osteoconnective tissue. We examine the phosphorylation analysis method by measuring the infrared absorption peak of phosphate group that observed at about 1070 cm^-1 (9.4 mm) with Fourier Transform Infrared Spectrometer (FT-IR). This result indicates that it is possible to identify a phosphorylation by measuring the infrared absorption peak of phosphate group observed at about 1070 cm^-1 with FT-IR method. And we examine laser-dephosphorylation using Free Electron Laser (FEL) as a novel dephosphorylation method. After irradiation of 9.4mm-FEL, infrared absorption peak of phosphate group is reduced. It is suspected that this lowering of the peak of phosphate group is the effect like dephosphorylation. These novel methods can be applied to quality control technology in regenerative medicine.

Citation Download Citation

Katsunori Ishii, Kaoru Yanase, Sachiko Suzuki-Yoshihashi, Masaya Yamamoto, Kunihiro Chihara, Yasuhiko Tabata, and Kunio Awazu "FT-IR analysis of phosphorylation and laser-dephosphorylation process", Proc. SPIE 5695, Optical Interactions with Tissue and Cells XVI, (15 April 2005); https://doi.org/10.1117/12.591904

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