High-throughput scanning with single-molecule sensitivity

Max Sonnleitner; Gunter Freudenthaler; Jan Hesse; Gerhard J. Schuetz

doi:10.1117/12.590292

29 March 2005 High-throughput scanning with single-molecule sensitivity

Max Sonnleitner, Gunter Freudenthaler, Jan Hesse, Gerhard J. Schuetz

Proceedings Volume 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III; (2005) https://doi.org/10.1117/12.590292
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We report on the application of a novel fluorescence-microscope based scanning device with single molecule sensitivity to microarray readout. The device is based on a CCD camera operated in time delay and integration (TDI) mode synchronized with the movement of a sample scanning stage, enabling continuous data acquisition. The implementation of a focus hold system keeps the sample in focus during scanning. Results from ultra-sensitive high-resolution microarray readout provide clear evidence for the superiority of the novel detection method over conventional microarray readout systems in particular regarding to sensitivity and dynamic range. Minute sample amount and lacking amplification methods will demand for this ultra-sensitive readout technology in future genomic and proteomic research.

Citation Download Citation

Max Sonnleitner, Gunter Freudenthaler, Jan Hesse, and Gerhard J. Schuetz "High-throughput scanning with single-molecule sensitivity", Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); https://doi.org/10.1117/12.590292

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