Investigating gradient sensing in cells through optical micromanipulation

Wolfgang Losert; Cory Poole; Ron Skupsky

doi:10.1117/12.589648

29 March 2005 Investigating gradient sensing in cells through optical micromanipulation

Wolfgang Losert, Cory Poole, Ron Skupsky

Proceedings Volume 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III; (2005) https://doi.org/10.1117/12.589648
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The aim of our work is to develop new optical tools to quantify parameters that may enter into models of cell motion in response to chemical gradients (chemotaxis). Dictyostelium discoidium is a well-known model organism for studying chemotaxis. We have developed a technique for manipulating Dictyostelium cells directly using a holographic laser tweezer array. Using this technique we have perturbed crawling Dictyostelium cells by changing their direction of motion. After tens of seconds, the cells generate protrusions perpendicular to the rotated polarization as they reorient in the direction of the local cAMP gradient. Here we describe how such micromanipulation may be used to test proposed biochemical pathways and their connection to mechanical deformations.

Citation Download Citation

Wolfgang Losert, Cory Poole, and Ron Skupsky "Investigating gradient sensing in cells through optical micromanipulation", Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); https://doi.org/10.1117/12.589648

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available