Surface-enhanced Raman spectroscopic detection of cancer biomarkers in intact cellular specimens

Dominic Omar Ansari; Douglas A. Stuart; Shuming Nie

doi:10.1117/12.591178

29 March 2005 Surface-enhanced Raman spectroscopic detection of cancer biomarkers in intact cellular specimens

Dominic Omar Ansari, Douglas A. Stuart, Shuming Nie

Proceedings Volume 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III; (2005) https://doi.org/10.1117/12.591178
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We have developed a system for the direct spectroscopic identification of protein biomarkers in biological samples. By conjugating Raman dyes and biomolecular targeting agents to gold nanoparticles, we have produced highly selective optically encoded probes. The nanostructures are grown within a cellular sample to generate a surface that is highly active for surface enhanced Raman spectroscopy (SERS). Both in vitro characterizations of SERS detection as well as the sensitive and specific detection of cancer biomarkers in cultured cancer cells have been demonstrated.

Citation Download Citation

Dominic Omar Ansari, Douglas A. Stuart, and Shuming Nie "Surface-enhanced Raman spectroscopic detection of cancer biomarkers in intact cellular specimens", Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); https://doi.org/10.1117/12.591178

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