Implantable fluorescence-based glucose sensor development

Bennett L. Ibey; Vamsi K. Yadavalli; Hope R. Thomas; Rebecca M. Rounds; Michael V. Pishko; Gerard L. Cote

doi:10.1117/12.585297

7 March 2005 Implantable fluorescence-based glucose sensor development

Bennett L. Ibey, Vamsi K. Yadavalli, Hope R. Thomas, Rebecca M. Rounds, Michael V. Pishko, Gerard L. Cote

Proceedings Volume 5702, Optical Diagnostics and Sensing V; (2005) https://doi.org/10.1117/12.585297
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

An implantable sensor is being created that allows measurement of blood glucose through fluorescent detection of an embedded chemical assay. The sensor is based on the competitive binding reaction between the protein Concanavalin A and various saccharide molecules, specifically a glycodendrimer and glucose. Previous studies have shown the ability of an embedded chemical assay using Con A and dextran with shorter wavelength dyes to both sense changes in glucose and generate sufficient fluorescent emission to pass through the dermal tissue. However, due to the chemical constituents of the assay, multivalent binding was evident resulting in poor spectral change due to glucose within the biological range. Use of a glycodendrimer and longer wavelength dyes has improved the sensor’s spectral change due to glucose and the overall signal to noise ratio of the sensor. In this work, a description of this sensor and the results obtained from it will be presented showing a large dynamic range of fluorescence with glucose.

Citation Download Citation

Bennett L. Ibey, Vamsi K. Yadavalli, Hope R. Thomas, Rebecca M. Rounds, Michael V. Pishko, and Gerard L. Cote "Implantable fluorescence-based glucose sensor development", Proc. SPIE 5702, Optical Diagnostics and Sensing V, (7 March 2005); https://doi.org/10.1117/12.585297

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