A common-path phase-shift interferometry surface plasmon imaging system

Y.-T. Su; Shean-Jen Chen; T.-L. Yeh

doi:10.1117/12.592532

31 March 2005 A common-path phase-shift interferometry surface plasmon imaging system

Y.-T. Su, Shean-Jen Chen, T.-L. Yeh

Proceedings Volume 5703, Plasmonics in Biology and Medicine II; (2005) https://doi.org/10.1117/12.592532
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

A biosensing imaging system is proposed based on the integration of surface plasmon resonance (SPR) and common-path phase-shift interferometry (PSI) techniques to measure the two-dimensional spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR phase imaging system can offer high resolution and high-throughout screening capabilities to analyze microarray biomolecular interaction without the need for additional labeling. With the long-term stability advantage of the common-path PSI technique even with external disturbances such as mechanical vibration, buffer flow noise, and laser unstable issue, the system can match the demand of real-time kinetic study for biomolecular interaction analysis (BIA). The SPR-PSI imaging system has achieved a detection limit of 2×10^-7 refraction index change, a long-term phase stability of 2.5x10^-4π rms over four hours, and a spatial phase resolution of 10^-3 π with a lateral resolution of 100μm.

Citation Download Citation

Y.-T. Su, Shean-Jen Chen, and T.-L. Yeh "A common-path phase-shift interferometry surface plasmon imaging system", Proc. SPIE 5703, Plasmonics in Biology and Medicine II, (31 March 2005); https://doi.org/10.1117/12.592532

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